Birrell, James (2026) Data for Opuku Djan et al. (2026) Catalysis Science & Technology. [Data Collection]
Birrell, James (2026) Data for Opuku Djan et al. (2026) Catalysis Science & Technology. [Data Collection]
Birrell, James 2026. Data for Opuku Djan et al. (2026) Catalysis Science & Technology. [Data Collection]. Colchester, University of Essex. 10.5526/erdr-00000258
Collection description
Raw electrochemistry data corresponding to the cyclic voltammograms and background subtracted peaks presented in the manuscript and SI.
| Item Type: | Data Collection | ||||||||
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| Division: | Faculty of Science and Health > Life Sciences, School of | ||||||||
| Subjects: | Q Science > QD Chemistry | ||||||||
| Keywords: | Hydrogenase, Electrochemistry, Redox potential | ||||||||
| Research funder: | n.a, n/a, n/a | ||||||||
| Grant reference: | n/a | ||||||||
| Grant title: | n/a | ||||||||
| Depositor: | James Birrell | ||||||||
| Date Deposited: | 23 Oct 2026 09:42 | ||||||||
| Last Modified: | 23 Oct 2026 09:57 | ||||||||
| URI: | http://researchdata.essex.ac.uk/id/eprint/258 |
| Collection period: |
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| Geographic area: | Leicester | ||||
| Country: | United Kingdom | ||||
| Data collection method: | Protein film voltammetry (PFV) and film preparation took place in a N2 atmosphere glovebox (Belle Technology Ltd, O2 < 2 ppm). All measurements were conducted at room temperature, using a square basal-plane pyrolytic graphite electrode (square BPPGE; geometric area of 0.09 cm2) as the working electrode in mixed buffer (15 mM sodium 4-(2-hydroxyethyl)-1-piperazineethanesulfonate (Na-HEPES, 15 mM sodium 2-(N-morpholino)ethanesulfonate (Na-MES), 15 mM sodium 2-(N-cyclohexylamino)ethanesulfonate (Na-CHES), 15 mM sodium tris(hydroxymethyl)methylamino]propanesulfonate (Na-TAPS) and 15 mM sodium acetate). The pH was adjusted to the required value (pH 3.0 – 13.0), using NaOH and HCl. A saturated calomel electrode (SCE, Hg/HgCl2 in saturated potassium chloride, KCl) was used as a reference and a platinum wire was used as the counter electrode. Electrochemical measurements were performed using an EmStat4R [SNS] potentiostat (PalmSens) operated through PSTrace software. Signal processing, fitting and baseline subtraction was performed using QSoas software. The corrections for this study were ⁓ +244 mV. Before protein adsorption, the PGE was polished with P2500 grit sandpaper (Kemet) and rinsed, then 2 μL of ≈0.5 mM protein was applied onto the electrode surface and was left to dry for ~ 5 min to form a film. The electrode was subsequently transferred to the electrochemical cell to carry out the electrochemical measurements. | ||||
| Data type: | Text | ||||
| Metadata language: | English | ||||
| Resource language: | English |
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