258 12 1581 3 6696 document 1581 Data for Opuku Djan 2026 Catal Sci Technol.zip application/zip 1b904add1eafec3eb6f803c8b44e3dfa MD5 335687 2026-09-23 17:26:44 https://researchdata.essex.ac.uk/258/1/Data%20for%20Opuku%20Djan%202026%20Catal%20Sci%20Technol.zip 258 1 1 application/zip archive Data for Opuku Djan 2026 Catal Sci Technol en public cc_by_4

Data for Opuku Djan 2026 Catal Sci Technol.zip

archive 73 disk0/00/00/02/58 2026-10-23 09:42:30 2026-10-23 09:57:02 2026-10-23 09:42:30 data_collection show Raw electrochemistry data corresponding to the cyclic voltammograms and background subtracted peaks presented in the manuscript and SI. Hydrogenase Electrochemistry Redox potential QD dep_bio Birrell James james.birrell@essex.ac.uk Text 2025-05-07 2026-09-01 Leicester Protein film voltammetry (PFV) and film preparation took place in a N2 atmosphere glovebox (Belle Technology Ltd, O2 < 2 ppm). All measurements were conducted at room temperature, using a square basal-plane pyrolytic graphite electrode (square BPPGE; geometric area of 0.09 cm2) as the working electrode in mixed buffer (15 mM sodium 4-(2-hydroxyethyl)-1-piperazineethanesulfonate (Na-HEPES, 15 mM sodium 2-(N-morpholino)ethanesulfonate (Na-MES), 15 mM sodium 2-(N-cyclohexylamino)ethanesulfonate (Na-CHES), 15 mM sodium tris(hydroxymethyl)methylamino]propanesulfonate (Na-TAPS) and 15 mM sodium acetate). The pH was adjusted to the required value (pH 3.0 – 13.0), using NaOH and HCl. A saturated calomel electrode (SCE, Hg/HgCl2 in saturated potassium chloride, KCl) was used as a reference and a platinum wire was used as the counter electrode. Electrochemical measurements were performed using an EmStat4R [SNS] potentiostat (PalmSens) operated through PSTrace software. Signal processing, fitting and baseline subtraction was performed using QSoas software. The corrections for this study were ⁓ +244 mV. Before protein adsorption, the PGE was polished with P2500 grit sandpaper (Kemet) and rinsed, then 2 μL of ≈0.5 mM protein was applied onto the electrode surface and was left to dry for ~ 5 min to form a film. The electrode was subsequently transferred to the electrochemical cell to carry out the electrochemical measurements. English English website Birrell James james.birrell@essex.ac.uk 2026-09-23 University of Essex Birrell James james.birrell@essex.ac.uk 10.5526/erdr-00000258 n/a n/a n.a n/a n/a United Kingdom