This is the data associated with with the above paper "Hands-free Photosynthesis: Autonomous Leaf-Level Multi-Chamber Gas Exchange System" comprising of data for figures and supplementary figures. Excel files contain leaf level gas exchange measurements collected from from LI-COR instrument and a novel automated photosynthesis system and whole plant transpiration data from a PlantArray system on sugarcane and cowpea, collected at The University of Illinois. Data collected in 2025.